Stem Cell and Cancer Research


The Role of Mesenchymal Stem Cells on Regulating PDGF and Islet Cells in Diabetes

See original article in Brawijaya University : https://jkb.ub.ac.id/index.php/jkb/article/download/2274/658

ABSTRACK

Diabetes is a group of metabolic disease characterized by increased level of blood glucose as a result of defect in β Cells destruction and or insulin resistance. Clinical studies showed that transplantation of islet is sufficient cure to relief from the diabetes-related symptom. However, the lack of applicable donor is becoming a serious problem.

Recent study has shown that Mesenchymal Stem Cells (MSCs) has high-potential to regenerate pancreatic tissue damage including islet Cells by involving various growth factors such as PDGF. The aim of this study is to determine the effect of umbilical cord MSCs in increasing the number of islet Cells and PDGF level appearance on the 44th day of remodeling phase in pancreatic tissue damage.

This study used 20 streptozotocin-induced balb-c mice (Mus musculus) to diabetes divided into four group (n=5/group) consisting control (K) and treatment (P). The control group was treated PBS while P1, P2, and P3 treated 5 5 5 different MSCs dose intraperitoneally (P1=1.5×10 , P2=3×10 , dan P3=6×10 Cells). Day 44, ELISA essay were performed using blood samples to identify PDGF level apearance and tissue histopathology for counting pancreatic islet Cells.

The result of this study presented the increased of islet sells significantly (p<0.05) and PDGF level appearance similar to the control. Here, our experimental in model of diabetic-induced balb-c mice (Mus musculus) suggested that MSCs is able to increase the number of islet cells and regulated PDGF level.

Keyword: Diabetes, islet cells, MSCs, PDGF level

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