Abstract
Introduction: Breast cancer has been shown to have the ability to survive by proliferation. Typhonium flagelliforme (Lodd), known as keladi tikus has ability of anti-proliferation on the murine P388 leukemia cell and NCI-H23 non-small cell lung carcinoma cell line, depend on the polarity of solvent and structure of cancer cell. The aim of this study was to compare the effectiveness of tuber and leave extract of Typhonium flagelliforme to inhibit the cell proliferation of MCF-7 breast cancer cell line.
Methods: in-vitro experimental study on the MCF-7 cell “Post test control group only design” the cell lines were divided into 49 groups that include the control group, 3 groups treated with DMC, ethanolic, hexanolic extract of Typhonium flagelliforme and 3 groups treated with DCM, ethanolic , and hexanolic extract of the leave of Typhonium flagelliforme at concentration of 1000, 500, 250, 125, 62,5, 31,25, 15,62, 7,81µg/m. Cell proliferation was estimated by MTT assay, log probit was used for the statistical analysis.
Results: DMC extract of the tuber of Typhonium flagelliforme inhibit the cell proliferation at IC5o = 63.08 µg/mL (nilai p<0.001), and the DMC extract of the leave of Typhonium flagelliforme inhibit cell proliferation at IC5o = 68.65 µg/mL.
Conclusion: The tuber extract of Typhonium flagelliforme is a better potent inhibitor of proliferation in the MCF-7 breast cancer cell line compared to the leave extract. J Indon Med Assoc. 2012:62;10-5
Keywords: antiproliferation, Typhonium flagelliforme, MTT Method, IC50
