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Real Time qRT-PCR

Real-Time Quantitative Reverse Transcription PCR (qRT-PCR) is a major development of PCR technology that enables reliable detection and measurement of products generated during each cycle of PCR process. This technique became possible after introduction of an oligonucleotide probe which was designed to hybridize within the target sequence. Cleavage of the probe during PCR because of the 5′ nuclease activity of Taq polymerase can be used to detect amplification of the target-specific product.